Widespread Transcription in an Amphibian Oocyte Relates to Its Reprogramming Activity on Transplanted Somatic Nuclei
نویسندگان
چکیده
منابع مشابه
Nuclear transplantation: reprogramming of transplanted nuclei.
Recent results in animal cloning have demonstrated that the events of cell differentiation can be reversed. This reversal necessarily requires genetic reprogramming of the transplanted nucleus. It has emerged from review articles that synthesis of nuclear and ribosomal RNA is blocked after fusion independently of cell cycle stage of the transferred nucleus, and that the cytoplasm condition of t...
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Nuclear transfer to oocytes is an efficient way to transcriptionally reprogram somatic nuclei, but its mechanisms remain unclear. Here, we identify a sequence of molecular events that leads to rapid transcriptional reprogramming of somatic nuclei after transplantation to Xenopus oocytes. RNA-seq analyses reveal that reprogramming by oocytes results in a selective switch in transcription toward ...
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Nuclear transfer to eggs or oocytes provides a potential route for cell-replacement therapies because oocytes directly reprogram transplanted mammalian somatic-cell nuclei such that they have an embryo-like pattern of gene expression. This includes a large increase in the mRNA level of the stem-cell marker gene oct4. We have developed a novel procedure to identify new proteins that greatly incr...
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Transplantation into eggs or oocytes is an effective means of achieving the reprogramming of somatic cell nuclei. We ask here whether the provision of gene-specific transcription factors forms part of the mechanism by which a gene that is repressed in somatic cells is transcribed in oocytes. We find that M1 oocytes have an extremely strong transcription-inducing activity. They cause muscle gene...
متن کاملI-10: Transcriptomics in Oocyte Mediated Cellular Reprogramming
a:4:{s:10:"Background";s:1707:"Early embryonic development in mammals begins in transcriptional silence with an oocyte-mediated transcriptional reprogramming of parental gametes occurs during a so called across-the-board process of “erase-and-rebuild”. In this process, the parental transcription programs are erased long before (maternal) or soon thereafter (paternal) fertilization to generate a...
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ژورنال
عنوان ژورنال: Stem Cells and Development
سال: 2012
ISSN: 1547-3287,1557-8534
DOI: 10.1089/scd.2011.0162